Expression of a Polygalacturonase Associated with Tomato Seed Germination
نویسندگان
چکیده
منابع مشابه
Expression of an expansin is associated with endosperm weakening during tomato seed germination.
Expansins are extracellular proteins that facilitate cell wall extension, possibly by disrupting hydrogen bonding between hemicellulosic wall components and cellulose microfibrils. In addition, some expansins are expressed in non-growing tissues such as ripening fruits, where they may contribute to cell wall disassembly associated with tissue softening. We have identified at least three expansi...
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Virus-induced gene silencing (VIGS) was used as a tool for functional analysis of cell wall-associated genes that have been suggested to be involved in leaf abscission. Tobacco rattle virus is an effective vector for VIGS in tomato (Lycopersicon esculentum). Silencing was more efficient when the plants were grown at 22 degrees C than when they were grown at 20 degrees C or 25 degrees C. The pho...
متن کاملAnalysis of tomato polygalacturonase expression in transgenic tobacco.
Tomato polygalacturonase is a cell wall enzyme secreted in large amounts during tomato fruit ripening. Polygalacturonase is synthesized as a glycoprotein precursor that undergoes numerous cotranslational and post-translational processing steps during its maturation, yielding three isozymes in tomato fruit, PG1, PG2A, and PG2B. To investigate the physiological roles of the three isozymes and the...
متن کاملDevelopmental expression and perturbation of arabinogalactan-proteins during seed germination and seedling growth in tomato.
Arabinogalactan-proteins (AGPs) are a family of highly glycosylated hydroxyproline-rich glycoproteins present throughout the plant kingdom. A synthetic chemical reagent, (beta-D-Gal)3 Yariv reagent, specifically binds AGPs and can be used for histochemical staining, isolating and probing the function of AGPs. Here, the role of AGPs in tomato (Lycopersicon esculentum Mill. cv. UC82B) seed germin...
متن کاملTwo tomato expansin genes show divergent expression and localization in embryos during seed development and germination.
Expansins are plant proteins that can induce extension of isolated cell walls and are proposed to mediate cell expansion. Three expansin genes were expressed in germinating tomato (Lycopersicon esculentum Mill.) seeds, one of which (LeEXP4) was expressed specifically in the endosperm cap tissue enclosing the radicle tip. The other two genes (LeEXP8 and LeEXP10) were expressed in the embryo and ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1999
ISSN: 1532-2548,0032-0889
DOI: 10.1104/pp.121.2.419